Effects of LRIG2 on proliferation of human glioma cells by RNA interference and the mechanism

Objective To study the effects of LRIG2 on the proliferation of glioma cells by RNA interference and the mechanisms underlying such effects.Methods The plasmid pGenesi12-LRIG2-shR-NA (siRNA) was transfected into GL15 glioma cells by Metafeetine, and the cells (siRNA) that had a stably suppressive LRIG2 expression were selected by G418.The control cells were transfected with scram-bled shRNA (scr).The changes in LRIG2, and epidermal growth factor receptor (EGFR) protein levels were measured by RT-PCR and Western blot.Growth curves were determined by the methyl thiazolyl tet-rasolium (MTT) assay.Cell cycles were analyzed by flow eytometry.Results The LRIG2 mRNA and pro-tein levels in pGenesi12-LRIG2-shRNA (siRNA) transfected cells were significantly lower than in scram-bled shRNA (ser) transfected cells.The EGFR protein level in pGenesil2-LRIG2-ShRNA (siRNA) cell was lower than in ser cells.The siRNA cells had a lower proliferation rate than ser cells.Cell cycle analysis showed that silencing LRIG2 caused G0/G1 cell cycle arrest.Conclusion Silencing LRIG2 expression could result in degradation of EGFR, then affect the downstream pathway of EGFR signaling, further sup-press the proliferation of GL15 cell and arrest the cell cycle in G0/G1 phase. Key words: LRIG2 protein,human;  Receptor,epidermal growth factor;  Cell proliferation; Cell cycle