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Inducible Nitric Oxide Synthase Gene Expression in Neonatal Rats Contribute to Hypoxic-Ischemic Brain Damage

初桂兰, 李艳芸, 程佶

Abstract

目的研究新生大鼠缺氧缺血性脑损伤(HIBD)时诱导型一氧化氮合酶(iNOS)mRNA的表达变化.方法制备新生大鼠左脑HIBD的模型.用反转录聚合酶链反应(RT-PCR)检测HIBD后6 h、24 h、48 h、5 d不同时点脑皮层iNOS mRNA的表达情况.经凝胶成像及分析系统扫描RT-PCR产物,用内参半定量分析iNOS mRNA的动态变化.同时观察光镜下神经元坏死变化.结果 7日龄Wistar大鼠对照组没有iNOS mRNA表达,HIBD 6 h开始表达,HIBD 24～48 h为表达高峰(与HIBD 6 h相比有显著性差异,P＜0.01),此后逐渐下降,HIBD 5 d仍可检测出(与HIBD 6 h相比有显著性差异,P＜0.01).观察光镜下神经元坏死在HIBD后24～48 h最显著.结论新生大鼠HIBD可诱导iNOS基因表达,其可能参与HIBD后的神经毒性损伤.

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Key concepts: Nitric oxide synthase, Nitric oxide, Gene, Gene expression, Hypoxia (environmental), Brain damage, Neuroscience, Biology