Protection of NF - κB activation during sevoflurane preconditioning in rat model of myocardial ischemia - reperfusion

Objective To research the activation of NF - κB during sevoflurane preconditioning on rat myocardium against ische-mia/reperfusion injury in vivo. Methods Forty - eight male SD rats were randomly divided into six groups of 8 animals each: ① Control group, Rats were experienced myocardium ischemia -reperfusion merely; ② Dimethylsulfoxide (DMSO) group, Dissolvent DMSO was administered intraperitoneaUy (IP);③ Parthenolide (PTN) group, Nuclear Factor-κB (NF- κB) inhibitor PTN (500μg/kg) was administered IP;④ Sevoflurane group, Rats received 2.5% sevoflurane for 30 rain and 15 min wash - out followed by a 30 minutes occlusion;⑤ FTN + sevoflurane group, FIN was administered If) 15 rain before exposure to sevoflurane;⑥ Sevoflurane + PTN group, FTN was administered IP after sevoflurane preconditioning. Tissue was obtained after 2 h reperfusion. Myocardial infarct sizes were determined using triphenyhetrazolium chloride staining. Results During sevoflurane administered for 30 min, there was statistically difference in HR, MAP and RPP among sevoflurane group, PTN + sevoflurane group and sevoflurane + PTN group (P < 0.05 ) and during reperfusion 1 h and 2 h, there was statistically difference in MAP and RPP among all groups (P <0.05). Sevoflurane group significantly (P < 0.05 ) reduced infarct size from (52.48 ～ 6.04 ) % [ control ( mean±SD) ] to ( 33.73±5.72 ) % ( P < 0. 05 ).Compared to Sevoflurane group ( 33.73±5.72) % , FIN + Sevoflurane group ( 52.60±5. 01 ) % and Sevoflurane + PTN group (52.39±7.00) % had statistical difference ( P < 0.05 ). Conclusion NF - κB might participate to protective mechanism of sevoflurane preconditioning. Key words: sevoflurane; preconditioning; ischemia/reperfusion injury; myocardium; NF-κB