The effect of sevoflurane postconditioning combined with sevoflurane preconditioning on plasma concentrations of SOD and MDA in rats with acute myocardium ischemia reperfusion

Objective By means of myocardial ischemia reperfusion model,to investigate whether sevoflurane postconditioning combined with sevoflurane preconditioning can reduce reperfusion injury and the protective effect is better than that of single treatment,and to explore its action mechanism in order to provide experimental basis for clinical application.Methods Fifty healthy male Wistar rats weighed 250~280g were randomly divided into 5 groups(n=10 in each group).In anesthetized open-chest rats,the left anterior descending artery was occluded for 30 minutes and reperfused for 120 minutes.Sham operation group(group S):placement of the silk suture under LAD without other intervention.I/R group:the rats received no other intervention except for before or after LAD occlusion.Sevoflurane preconditioning group(sevopre group):the rats were given with 2.5% sevoflurane 30min before occlusion for 15min,and washed out for 15min,the other treatment was similar to those of I/R group;Sevoflurane postconditioning group(sevopo group):administing 2.5 % sevoflurane 1 min before reperfusion for 5min;Sevoflurane postconditioning combined with sevoflurane preconditioning group(pre+po group):administrating 2.5% sevoflurane 30min before occlusion for 15min,and washed out for 15min,and administrating 2.5% sevoflurane 1min prior to reperfusion for 5min.At the end of the experiment,the carotid arterial blood and myocardium samples were got to detect myocardial infarct area,the levels of SOD and activity of MDA.Results 120min after reperfusion,infarct size(22.69±3.97) was significantly decreased(P0.05),the superoxide dismutase(SOD) activity was markedly increased,however,MDA levels were obviously decreased(P0.05) in po + pre group,as compared with those in single treatment group.Conclusion The protective effect of preconditioning combined with postconditioning with sevoflurane against myocardial ischemia reperfusion injury is probably achieved by increasing SOD activity and decreasing MDA content.