The preparation and trait study of gelatin microspheres by new non-toxic crosslinking technology

Objective To introduce a new type of preparation of gelatin microspheres by new non-toxic crosslinked technique. Methods The gelatin microspheres were prepared by non-toxic crosslinking technology and nano micro-channel cutting technology.100 gelatin microspheres were randomly selected as the experimental group, while 100 Embosphere microspheres were selected as the control group. Under the microscope, the shape and diameter of gelatin microspheres was evaluated. Results The gelatin microspheres of the experimental group were spherical shape, and its particle size range from 30 μm to 1 000 μm. The sample particle size was(197.90±5.39) μm, and particle size distribution was normal. The gelatin microspheres of the control group were also spherical shape, and its particle size range from 100 μm to 700 μm. The sample particle size was(200.40±12.73) μm, and particle size distribution was approximately normal. Conclusion The independent development's nano micro-channel cutting technology was advanced and the productivity of microspheres was high, and the particle size was uniform. However, this study has beed in the early stage, and it still need animal experiments which can test biocompatibility and embolic effect. Key words: Gelatin microspheres; Genipin; Non-toxic crosslinking