2019•Zhonghua shiyan waike zazhiRequires access
In vitro and in vivo effects of RNA interference targeting c-Myc gene on gastric cancer SGC7901 cells
Tao Zeng, Wen Jian-hu, Jixiang Wang
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Abstract
Objective
To observe the effect of RNA interference to c-Myc on proliferation of gastric cancer SGC7901 cells in vitro and in vivo.
Methods
The c-Myc gene of gastric cancer SGC7901 was silenced by RNA interference technique. The expression of c-Myc gene of SGC7901 was detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR). The proliferation of gastric cancer SGC7901 was detected by methyl thiazol tetrazolium (MTT) assay and in vivo tumorigenesis.
Results
After the silencing of c-Myc gene by RNA interference technology, the proliferation rate and tumorigenic growth rate of gastric cancer SGC7901 decreased significantly (on day 7, compared with the blank group, the tumor inhibition rate of small interfering RNA (siRNA)-scramble group was 98.78% (P>0.05), while that of the experimental group was 49.96% (P<0.05).
Conclusion
After the c-Myc interference, gastric cancer SGC7901 in vitro and in vivo proliferation rate decreased.
Key words:
Gastric carcinoma; RNA interference; c-Myc; Proliferation