Observation of early seizure and hippocampus change in rats with traumatic brain injury

Objective To make model of early seizures after fluid percussion injury in brain of rats,to observe the expression of hippocampal glial fibrillary acidic protein (GFAP) and hippocampal mossy fibers,and to explore the early post-traumatic mechanism of epilepsy seizures.Methods Adult rats were divided into different groups.Experimental group was divided into three different sub-groups and fluid percussion brain injury model was made.GFAP in hippocampal area was detected on the 7th day after injury.Timm staining was carried out and observed under a microscope.Results Early seizures appeared in severe shock group.Average integral optical density of GFAP in the hippocampal region was 38.87 ± 1.24,and average integral optical density of Timm's staining granules was 1.38 ±0.10.As compared with control group,the difference was statistically significant (P ＜ 0.05).Conclusion The GFAP expression in the hippocampal region and mossy fiber spouting hyperplasia is significantly increased in rats with early seizures after injury,and closely related to the sensitivity of epileptic seizure. Key words: Early epilepsy;  Glial fibrillary acidic protein;  Mossy fiber