Injury in rat hippocampus kindled by amygdale and expression of glial fibrillary acidic protein

Objective To study the correlation between the injury in rat hippocampus after epilepsy kindled by amygdala and the expression of glial fibrillary acidic protein(GFAP),and the role of GFAP in experimental epilepsy.Methods The rat epilepsy model was set up by electron amygdala,the pathological changes in hippocampus after kindled epilepsy were observed by using optical microscope and electron microscope.And the number of GFAP immunoreactive positive cells in hippocampus after rat kindled epilepsy,area,circumference and integral optical density were detected by immunohistochemistry.Results By using optical microscope,the neurocytes of hippocampus in experimental group were observed to denaturalize,tumefy,destroy and disappear.And under electron microscope,the karyon was irregular,nucleoles deviated,chondriosome tumefied,crista collapsed,membrane was damaged,substance cavitated.Such changes were more evident with the increased paroxysm of epilepsy,the parameters between control group and operation group had no difference(P0.01).But make a monofactorial variance analysis among cells' number,area,circumference and integral optical density in different groups,it showed that there were significant differences between optional two groups(P0.01);at the same time,the more serious injury in hippocampus,the more powerful GFAP immunoreaction was.Conclusion The more serious injury in hippocampus,the more powerful GFAP immunoreaction is.The correlation between astrocyte and injury of brain after epilepsy can be speculated by detecting GFAP.