Influence of phosphatase of regenerating liver-3 on the biological behavior of prostate cancer LNCaP cells

Objective To investigate influence of phosphatase of regenerating liver-3 (PRL-3)gene silencing with small interference RNA (siRNA) on biological behaviors of human prostate cancer LNCaP cells,including proliferation and invasion.Methods The method of silencing PRL-3 was established by lentivirus-mediated RNAi.The cells were divided into 3 groups.In experimental group,the expression of PRL-3 in LNCaP cells was stably blocked by lentivirus-mediated RNAi.In negative control group,the cells were transfected with lentivirus-mediated control RNAi (without any interference to PRL-3).The normal LNCaP cells served as blank control group.Methyl thiazol tetrazolium (MTT) assay was used to measure the proliferation of LNCaP cells,and Transwell assay was used to observe invasion potency.Results The experimental group showed significantly lower levels of PRL-3 mRNA and protein than those in negative control and blank control groups after transfection (P ＜ 0.05).The invasion potency of LNCaP cells was significantly suppressed after transfection (P ＜ 0.05),but there was no significant difference among the three groups on proliferation.Conclusion Down-regulation of PRL-3 expression can weaken invasion potency of LNCaP cells,but it had no effect on proliferation. Key words: Prostate cancer;  Phosphatase of regenerating liver-3 ;  RNA interference