Biological characteristics of rabbit adipose-derived stem cells and their induction to osteogenesis

Objective To investigate the isolation and culture of adipose-derived stromal cells (ADSCs) and observe the biological characteristics and osteogenic differentiation potential of ADSCs.Methods The white adipose tissue was obtained from the inguinal fat pads of adult Japanese white rabbits,and digested with type Ⅰ collagenase.Primary ADSCs were passaged and cultured when the cells were about 80％ confluent.The ultrastructure and surface marker expression of ADSCs were observed under the inverted microscopy.The growth curve was made from the 3rd,5th,10th,and 15th generations of cells.The possibility of inducing ADSCs to osteogenesis in specific differentiation medium was observed.Results The growth curves of generations of cells had no significant diffenence,which displayed reversed S-shaped.Gomori and Von Kossa staining demonstrated postitive expression of ALP and formation of mineralized nods in extracellular matrix.The cultured ADSCs were positive for CD29 and CD44,and negative for CD34 and CD45.Conclusion The abundant ADSCs can be induced to differentiate into osteogenesis,and have stable biological characteristics.ADSCs may be served as seeding cells in the bone tissue engineering. Key words: Adipose-derived stem cells ;  Culture ;  Osteogenesis ;  Differentiation