Study on Isolation,Cultivation and Inducing to Adipogenesis,Osteogenesis of Rabbit Adipose-derived Stem Cells

Objective To isolate,cultivate adult rabbit adipose-derived stem cells (ADSCs),and to explored the possibility of inducing ADSCs to adipogenesis and osteogenesis in specific differentiation medium. Methods The adipose tissue was obtained from the inguinal fat pads of young adult New Zealand white rabbits. Primary ADSCs were isolated by the methods of collagenaseⅠdigestion subsequently and were expanded in monolayer with serial passages at confluence.The third passage of ADSCs was induced to differentiate after exposure to adipocytic or osteogenic medium.The Oil Red O staining, alkaline phosphatase(ALP)and Alizarin red S staining were used to detect the results.Results The cells isolated from subcutaneous adipose tissues of rabbits had a strong capability of proliferation.After cultured in adipogenic differentiation medium,the cells were Oil Red O staining positive.After cultured in osteogenic differentiation medium,the cells were alkaline phosphatase (ALP) and Alizarin red S staining positive.Conclusion ADSCs were successfully isolated and cultured from rabbit adipose tissue. These cells have adipogenic and osteogenic potential.This study showed that the ADSCs could be served as a seed cells in the bone tissue engineering.