Protective effect of sodium tanshinon II A on lung injury induced by limb ischemia-reperfusion in rats

Objective To investigate the protective effect of sodium tanshinone Ⅱ A on lung injury induced by limb ischemia-reperfusion in rats. Methods 60 SD rats were randomly divided into three groups:control group (group A,n =20),limb-ischemia-reperfusion group (group B,n =20),and tanshinone Ⅱ A group (group C,n =20). The levels of malondialdehyde (MDA),superoxide dismutase (SOD),xanthine oxidase (XOD),interleukin-8 (IL-8),and tumor necrosis factor-α (TNF-α) in plasma and lung tissue were measured. The levels of TXB2 and 6-kcto-PGF1α in serum were measured by radioimmunoassay. The morphological and ultrastructure changes of lung tissues were observed under light microscope and electron microscope,respectively. Results ①Under light microscope,compared with group B,expansion of pulmonary vascular,aggregation of inflammatory cell,pulmonary edema relieved in group C. Under electron microscopy,compared with group B,mitochondrial swelling or degeneration,edema of basement membrane,swelling of pulmonary capillary endothelial cell,and pulmonary interstitial edema relieved in group C. ②MDA and XOD in plasma and lung tissue of group B were significantly higher than those in group A (all P ＜0. 01),and those in group C were significantly lower than those in group B (all P ＜0. 01). SOD in plasma and lung tissue of group B was significantly lower than that in group A (all P ＜0. 01). and that in group C was significantly higher than that in group B (all P ＜0. 01). ③IL-8、TNF-α in serum and lung tissue of group B were significantly higher than those in group A (all P ＜0. 01),those in group C were lower than those in group B ( P ＜0. 05 or P＜0. 01),and there was no statistical significance between group C and group A. ④TXB2 and 6-keto-PGF1α in serum of group B were significantly higher than those in group A (all P ＜0. 01),and those in group C were significantly lower than those in group B (all P ＜0. 01). TXB2 in serum of group C was higher than that in group A ( P ＜0. 05). There was no statistical significance on serum 6-keto-PGF1α between group C and group A. Conclusions Tanshinone Ⅱ A can alleviate lung injury of rats with limb ischemia-reperfusion. Key words: Lung; Ischemia-reperfusion injury; Tanshinion ⅡA; Rats