2017•Unpublished venueRequires access
The etiological characteristics of a gastroenteritis outbreak caused by norovirus in Leshan city of Sichuan province
Xiaozhen Ma, Xia Chen, Ranran Cao, Wenhui Zhong, Xie Ying-Guo, Na Chen, Zhenyan Chen
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Abstract
Objective
To investigate the genotypes and genetic characteristics of the pathogens associated with an outbreak of acute gastroenteritis in Leshan city, Sichuan province.
Methods
Three stool samples and 3 vomitus samples from an acute gastroenteritis outbreak and 6 stool samples from healthy children were collected. Norovirus nucleic acid was detected by real-time reverse transcription polymerase chain reaction. The positive samples were amplified by conventional reverse transcription-polymerase chain reaction and the amplicons were sequenced.
Results
Six samples from the acute gastroenteritis outbreak were positive for norovirus. Sequence alignment and phylogenetic analysis showed that these 6 strains of virus belonged to GⅡ.2 genotype.
Conclusions
This outbreak of acute gastroenteritis was caused by genotype GⅡ.2 norovirus.
Key words:
Norovirus; Gastroenteritis; Outbreak; Etiological characteristics