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2013•Chinese Journal of Health Laboratory TechnologyRequires access

Pathology research on an acute gastroenteritis outbreak in Changzhou

XU Xiao-yi

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Abstract

Objective:To determine the pathogen and genogroup of an acute gastroenteritis outbreak by laboratory diagnosis of suspected cases samples.Methods:Thirty-six samples were tested for Norovirus by Real-time PCR.Eight positive samples were amplified by reverse transcriotion PCR,and the products were sequenced,then genotype was identified and phylogenetic tree was constructed.Results:Norovirus GII was detected positively in 15 of 36 samples.Sequencing and phylogenetic analysis revealed that 8 strains of Norovirus belonged to GII/12.Conclusion:The epidemic event of acute gastroenteritis was caused by GII/12 Norovirus.

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Objective:To determine the pathogen and genogroup of an acute gastroenteritis outbreak by laboratory diagnosis of suspected cases samples.Methods:Thirty-six samples were tested for Norovirus by Real-time PCR.Eight positive samples were amplified by reverse transcriotion PCR,and the products were sequenced,then genotype was identified and phylogenetic tree was constructed.Results:Norovirus GII was detected positively in 15 of 36 samples.Sequencing and phylogenetic analysis revealed that 8 strains of Norovirus belonged to GII/12.Conclusion:The epidemic event of acute gastroenteritis was caused by GII/12 Norovirus.

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Available abstract

Objective:To determine the pathogen and genogroup of an acute gastroenteritis outbreak by laboratory diagnosis of suspected cases samples.Methods:Thirty-six samples were tested for Norovirus by Real-time PCR.Eight positive samples were amplified by reverse transcriotion PCR,and the products were sequenced,then genotype was identified and phylogenetic tree was constructed.Results:Norovirus GII was detected positively in 15 of 36 samples.Sequencing and phylogenetic analysis revealed that 8 strains of Norovirus belonged to GII/12.Conclusion:The epidemic event of acute gastroenteritis was caused by GII/12 Norovirus.

Key concepts: Norovirus, Outbreak, Acute gastroenteritis, Genotype, Phylogenetic tree, Virology, Pathogen, Biology

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