Protective effect of continuous hypothermic perfusion on transplanted liver in rats

Objective To explore the protective effect of continuous hypothermic perfusion on donor liver. Methods Sixty Wistar rats were randomly divided into the control group (sham operation group, n＝12), donor continuous hypothermic reperfusion group (n＝24) and static cold storage group (n＝24). The donor keeping time was 5 h. The samples of blood and hepatic tissue were col-lected 24 h after liver transplantation. Meanwhile, the bile flow during the 24 h was recorded. Results Compared with the control group, liver functional parameters were higher in other two groups (P＜0.05). It was significantly lower in the continuous hypothermic reperfusion group than in the static cold storage group (P＜0.05). Meanwhile, the bile flow in the continuous hypothermic reperfusion group was more than that in the static cold storage group (P＜0.05). While γ-GGT and the Glucose(Glu) in the bile of the continuous hypothermic reperfusion group were lower than those in the static cold storage group (P＜0.05). Conclusions Continuous hypothermic perusion of donor liver during the keeping time has protective effect on the liver after transplantation. Key words: Liver Transplantation;  Liver donor;  Hypothermic perfusion;  Rat