Change of reproductive activity of bone marrow mesenchymal stem cells in experimental acute necrotic pancreatitis rats

Objective To analyze the percentage and reproductive activity of MSCs on different time point after ANP induction.Methods Experimental ANP was induced by intra pancreatic duet injection of sodium taurocholate (STC).Animals were sacrificed 12,24 and 36 h after model induction.Bone marrow cells were implanted for primary culture.Colony formation rate of bone marrow cells was calculated by CFU-F method.At the end of primary culture,surface markers CD29,CD44,CD4s on bone marrow cells were detected with flow cytometry,and percentage of Lin-CD29+CD44+CD45-cells (MSCs) in all cells was calculated.Results Colony formation rates of ANP 12 h,24 h and 36 h group were 20.33± 8.73,17.62±5.65 and 8.89±3,92,respectively,significantly higher than that of control (3.83 ±1.47,P<0.05).Percentage of Lin-CD29+CD44+CD45-cells (MSCs) of ANP 12 h,24 h and 36 h group were (7.61±0.67)%,(6.43±0.54)% and (3.30±0.41)%,respectively.ANP 36 h group was significantly lower than (5.18±0.35)% of control group(P<0.05),while percentage of ANP 12 h and 24 h group was significantly higher than that of control group (P<0.05).Conclusions The percentage and reproductive activity of bone marrow MSCs have changed during the periods of ANP. Key words: Panereatitis; acute neerotizing; Mesenchymal stem cells; Rat; Cell proliferation