Determination of pretransplantational cell viability by MTT colorimetric assay

Objective To assess the role of MTT colorimetric assay in measuring mitochondrial succinate-dehydrogenase(SDH)and establish a new way to determine pretransplantational cell viability.Methods Human or rat testicular Leydig cell suspensions were incubated with MTT medium,isopropanol was used to dissolve MTT fornlazan,then the absorbance of supertants was measured at 563 nm wavelength.Cell culture and testosterone production were used to aaseas the reliability of MTT colorimetric assay with conlparimn with Trypan bluedye.Results 1 g/L MTT concentration,3 h incubation,and impropanol as a solvent were the best optimum for the cell culture in vitro and testosterone determination showed that MTT colorimetric assay was more sensitive and accurate than Trypan bluedye.MTT colorimetric assay was employed to successfully measure cell viability for 10 cases of human testicular Leydig cell transplantation.Conelusion MTT colorimetric assay provided a reliable,objective,accurate method for determining the pretransplantational cell viability. Key words: MTT colorimetric assay; Cell/viability; Cell/transplantation; Trypan btuedye