Study of the Biocompatibility Between Bone Morphogenetic Protein Microspheres and Bone Marrow Stem Cell
覃昱, Pei guo Xian, 谢德明, J.M. Dan⁎, 魏宽海
Abstract
覃昱, Pei guo Xian, 谢德明, J.M. Dan⁎, 魏宽海
Abstract
目的研究骨形态发生蛋白微球的生物相容性.方法将壳聚糖与海藻酸钠制成复合微球,包裹骨形态发生蛋白,采用体外细胞培养技术,将微球与细胞一起培养,通过对细胞增殖率及ALP活性、细胞微量蛋白的检测,观察骨形态发生蛋白微球对培养细胞的影响. 结果骨形态发生蛋白微球对骨髓基质细胞的增殖无明显影响,但对其分化及分泌功能均有明显促进作用.结论骨形态发生蛋白微球生物相容性良好,能够提高骨髓基质细胞的体外成骨能力.
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目的研究骨形态发生蛋白微球的生物相容性.方法将壳聚糖与海藻酸钠制成复合微球,包裹骨形态发生蛋白,采用体外细胞培养技术,将微球与细胞一起培养,通过对细胞增殖率及ALP活性、细胞微量蛋白的检测,观察骨形态发生蛋白微球对培养细胞的影响. 结果骨形态发生蛋白微球对骨髓基质细胞的增殖无明显影响,但对其分化及分泌功能均有明显促进作用.结论骨形态发生蛋白微球生物相容性良好,能够提高骨髓基质细胞的体外成骨能力.
Key concepts: Bone marrow, Biocompatibility, Stem cell, Microsphere, Bone morphogenetic protein 2, Bone Marrow Stem Cell, Bone morphogenetic protein, Biomedical engineering