2003•中国医师杂志Requires access

Study of the Biocompatibility Between Bone Morphogenetic Protein Microspheres and Bone Marrow Stem Cell

覃昱, Pei guo Xian, 谢德明, J.M. Dan⁎, 魏宽海

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Abstract

目的研究骨形态发生蛋白微球的生物相容性.方法将壳聚糖与海藻酸钠制成复合微球,包裹骨形态发生蛋白,采用体外细胞培养技术,将微球与细胞一起培养,通过对细胞增殖率及ALP活性、细胞微量蛋白的检测,观察骨形态发生蛋白微球对培养细胞的影响. 结果骨形态发生蛋白微球对骨髓基质细胞的增殖无明显影响,但对其分化及分泌功能均有明显促进作用.结论骨形态发生蛋白微球生物相容性良好,能够提高骨髓基质细胞的体外成骨能力.

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What this paper is about

目的研究骨形态发生蛋白微球的生物相容性.方法将壳聚糖与海藻酸钠制成复合微球,包裹骨形态发生蛋白,采用体外细胞培养技术,将微球与细胞一起培养,通过对细胞增殖率及ALP活性、细胞微量蛋白的检测,观察骨形态发生蛋白微球对培养细胞的影响. 结果骨形态发生蛋白微球对骨髓基质细胞的增殖无明显影响,但对其分化及分泌功能均有明显促进作用.结论骨形态发生蛋白微球生物相容性良好,能够提高骨髓基质细胞的体外成骨能力.

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Available abstract

目的研究骨形态发生蛋白微球的生物相容性.方法将壳聚糖与海藻酸钠制成复合微球,包裹骨形态发生蛋白,采用体外细胞培养技术,将微球与细胞一起培养,通过对细胞增殖率及ALP活性、细胞微量蛋白的检测,观察骨形态发生蛋白微球对培养细胞的影响. 结果骨形态发生蛋白微球对骨髓基质细胞的增殖无明显影响,但对其分化及分泌功能均有明显促进作用.结论骨形态发生蛋白微球生物相容性良好,能够提高骨髓基质细胞的体外成骨能力.

Key concepts: Bone marrow, Biocompatibility, Stem cell, Microsphere, Bone morphogenetic protein 2, Bone Marrow Stem Cell, Bone morphogenetic protein, Biomedical engineering

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