Effect of lidocaine on LPS-induced NF-KB activity in rat peritoneal macrophages

Objective To investigate the effect of lidocaine on the LPS-induced NF-kB activity in rat peritoneal macrophages. Methods The peritoneal macrophages obtained from male Wistar rats were placed in 12- well plates at 2 × 10 6 cell/ml after being cultured for 3 days. Each well contained 1 ml of cell suspension. The cells were randomized into control group （group C）, LPS group and 3 LPS ＋ lidocaine group S （group LL1,2,3 ） （n = 10 wells each） . In group LPS and LL1,2,3 , the cells were exposed to LPS 100 ng/ml. In group LL1,2,3 the cells were exposed to lidocaine 2, 20 200 kg/ml respectively in addition to LPS 100 ng/ml. After being incubated for 24 h, the HMGB1 concentration in the supematant （by ELISA） and HMGB1 mRNA expression （by RT-PCR） and NF-kB activity in the cells were measured. Results LPS significantly increased HMGB1 concentration, HMGB1 mRNA expression and NF-kB activity in the supernatant. Lidocaine treatment significantly attenuated the LPS-induced increase in HMGB1 concentration HMGB1 mRNA expression and NF-kB activity in a dose-dependent manner. Conclusion Lidocaine can inhibit NF-kB activity in the rat peritoneal macrophages and in turn inhibit the synthesis and release of HMGB1. Key words: Lidocaine ;  NF- kappa B ;  Endotoxemia ;  Macrophages