Effects of Lidocaine on the release and translocation of HMGB1 in macrophages induced by lipopolysaccharide

Objective To investigate the effects of Lidocaine on expression and translocation of the high mobility group box-l(HMGB1) in rat peritoneal macrophages induced by lipopolysaccharide(LPS).Methods Peritoneal macrophages obtained from Wistar rats and incubated in 12-well tissue culture plates for 2-3 days were divided into 5 groups:the control group,LPS group,Lidocaine 2 mg/L +LPS treatment group(L1+LPS treatment group),Lidocaine 20 mg/L +LPS treatment group(L2+LPS treatment group) and the Lidocaine 200 mg/L +LPS treatment group(L3+LPS treatment group).After 6,12,24 and 48 h treatment,the concentrations of HMGB1 in the cell culture medium were measured by ELISA.The translocation of HMGB1 in rat peritoneal macrophages was observed by cellular immunochemistry.Results After rat peritoneal macrophages were stimulated by LPS,the LPS group showed that the release of HMGB1 was increased at 12h and reached the peak at 24 h.Compared with the LPS group,Lidocaine treatment groups decreased in release of HMGB1 in various degrees especially in the 20 mg/L+LPS treatment group(P0.05).Translocation of HMGB1 from the cell nucleus to the cytoplasm in the macrophage was obviously suppressed.Conclusion Lidocaine(20 mg/L) obviously inhibits the release and translocation of HMGB1 in rat peritoneal macrophages induced by LPS.