2017•Chin J Clin Lab Mgt (Electronic Edition)Requires access
Analysis of HBV genotypes and mutation patterns of HBV P gene in Linfen
Linping Hou, Junying Yang, Hua Hao, Chunliang Shi, Yunhong Zhao
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Abstract
Objective
To study hepatitis B virus (HBV) genotype distribution and mutation patterns of the P region of HBV gene in Linfen.
Methods
Realtime quantitative PCR based on fluorescence probe was utilized to detect the genotypes of HBV epidemic strains in Linfen; and the P region of HBV that isolated from 40 patients with chronic HBV infection and with antiviral treatment was sequenced. The most common P area resistance gene mutation sites (rt169-173-180-181-184-202- 204-207-213-214-215-229-233-236-237-238-250) were analyzed.
Results
Of the HBV strains isolated from 272 patients with chronic hepatitis B, 256 were C genotype (94.1%) and 16 were of B genotype (5.9%), There was no B+C genotype and other genotypes. Compared with B genotype, the DNA quantification value of C genotype in clinical specimens was higher(t=6.83, P 0.05). Among 40 cases, HBV P area gene sequence was successful determinated. There were 2 rtM204V mutations, 2 rtM204I mutations, and 2 rtN238S mutations. All cases of gene mutation were single locus mutation and the mutation rate was 15.0% (6/40).
Conclusions
Genotype C is the dominant genotype of HBV in Linfen and only single locus mutations of the P region are detected in this study.
Key words:
Hepatitis B virus; Genotype; Drug resistance; Mutation