Construction of recombinant adenovirns vector coding the human thioredoxin gene

Objective To replicate defective recombinant adenoviruses coding the human thioredoxin （hTRX） gene by means of recombination. Methods The interest gene fragment was obtained by RT-PCR and sequencing. Its cDNA was cloned into the expression vector pshuttle to construct recombinant eukaryotic expression vector pshuttle-hTRX, whose transient expression in HeLa cell line was detected by immunochemistry and Western blot, recombinant adenoviruses adeno-hTRX was then constructed and amplified. Results The structure of the recombinant adenoviruses had been constructed and confirmed by light microscopy of 293 cell, PI-Sce I and I-Ceu I enzyme restriction analysis and PCR amplification. Conclusions The recombinant adenoviruses carrying thioredoxin can be used in the gene therapy for cardiovascular diseases. Key words: Gene therapy;  Adenovirus E1A proteins;  DNA, recombinat;  Thioredoxin