Detection of EGFR genemutations in patients with non-small cell lung cancer by denaturing high performanceliquid chromatography technology

：Objective To investigatethe advantages of detection for EGFR gene mutations by denaturing high performance liquidchromatography (DHPLC) technology. Methods DHPLC was used to detect EGFR gene mutations atexon 19 and 21 in 49 cases of non-small cell lung cancer (NSCLC) patients,and the directDNA sequencing was used to verify the accuracy of DHPLC detection. Results EGFR genemutation was identified from 13 of 49 cases by DHPLC,including deletion mutation at exon19 in 10 cases (76.92 %) and alternative mutations at exon 21 in 3 cases (23.08 %).Mutation results of DHPLC was consistent with DNA direct sequencing. The results of thedirect DNA sequencing were the same as those of DHPLC. The sensitivity of mutation test byDHPLC was 100 %. Conclusion DHPLC technology can be used for large scale screening of EGFRgene mutation with rapid and accuracy.