2008•中国医师杂志Requires access
Mononuclear cells were induced into endothelia progenitor cells by VEGF165 and bFGF
Ye-qin Fang, Xiumei Xie, Jin He, Xiaobing Chen, Xiuli Li
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Abstract
Objective To determine the biological traits and optimal condition for the induction and differentiation of endothelial progenitor cells from peripheral blood in healthy adults. Methods Mononuelear cells were isolated from peripheral blood of healthy adults by Ficoll-density eentrifugation. The isolated ceils were cultured in 1640 medium supplemented with VECF165 and bFGF. The EPC specific surface mark CD34 and KDR were assessed by fluorescence activated cell sorter(FACS)analysis: EPC were characterized as adherent cells double positive for DiL-acLDL uptake and lectin binding by direct fluorescent staining under a hser scanning confocal microscope. EPC migration were assayed by MTr assay. Result The number and migration ability of EPC were increased by VEGFl65 and bFGF. Conclusion Endothelial progenitors cells can be derived from mononuclear cells of peripheral blood at specific conditions.
Key words:
Vascular endothelial growth factors; Fibroblast growth factor 2; Monoeytes; Endothelial cells; Stem cells; Cell differentiation