Reseach of Isolation and Culture of Endothelial Progenitor Cells in Human Peripheral Blood

【Objective】To determine the isolation and culture of endothelial progenitor cells(EPC) from peripheral blood in healthy adults,and reseach cell shape,amount,activity and clusters.【Methods】Mononuclear cells isolated from peripheral blood of healthy adults by Ficoll-density centrifugation.The isolated cells were cultured in 1640 medium supplemented with VEGF and bFGF.The EPC specific surface mark CD34 and KDR were assessed by fluorescence activated cell sorter(FACS)analysis.EPC were characterized as adherent cells double positive for DiL-acLDL uptake and lectin binding by direct fluorescent staining under a laser scanning confocal microscope.EPC migration was assayed by MTT assay.【Results】The number and activity of endothelial progenitor cells cultured significantly increased.【Conclusion】Endothelial progenitors cells are cultured and differentiated,at specific conditions derived from mononuclear cells of peripheral blood.