Distribution and drug resistance profile of 280 strains of bacteria culture positive bacteria

Objective To analyze the clinical distribution and the drug resistance of pathogenic bacteria in the infectious department and to provide the evidence for clinical therapy. Methods Bacterial culture of all samples in infection department from January 2013 to December 2015. The species identification and drug sensitivity tests were performed using VITEK automatic systems, then the proportion of the strains and the distribution among the specimens and the results of sensitivity to antibiotics were analyzed. Results A total of 280 pathogens were isolated. In terms of the detection of the positive specimens, the three most common ones were in turn the blood (32.9%, n=92 ), the sputum (26.8%, n=75) and urine (11.4%, n=32). In the detection of strains, gram-negative bacilli accounted for 48.9%, gram positive bacteria accounted for 22.1%, fungi accounted for 28.9%. Among the gram negative bacteria, Escherichia coli accounted for 20.4%, klebsiella pneumonia accounted for 6.4%, acinetobacter baumannii accounted for 2.9%, brucella accounted for 12.9%. Among the gram positive bacteria, coagulase negative staphylococci accounted for 6.4%, enterococcus faecium accounted for 4.6%, staphylococcus aureus accounted for 3.9%, streptococcus accounted for 3.6%. Among the fungi, candida albicans accounted for 12.9%, candida krusei accounted for 2.1%, aspergillus accounted for 4.6%. In drug sensitivity test, the resistance rate of Escherichia coli to imipenem was 0%, klebsiella pneumoniae and acinetobacter baumannii to imipenem were 5.6% and 12.5%, no vancomycin and linezolid resistant Staphylococcus aureus was found, the resistant rate of enterococcus to vancomycin was 5.6%. The antibacterial activity of gram positive bacteria was poor by quinolones drugs, especially the drug resistance of enterococcus to quinolones was >94.0%. The resistance rate of streptococcus pneumoniae to penicillin was 10.0%. Conclusions Gram-negative bacteria are the major pathogens isolated from infection department, mainly obtained by blood culture, the bacteria sensitive antimicrobial spectrum is narrow, the drug resistance is serious. The drugs have high sensitivity to fungi. Key words: Infectious department; Pathogenic bacteria; Drug resistance