2015•Zhonghua xingwei yixue yu naokexue zazhiRequires access
Effects of enriched enviroment on neurons apoptosis in hippocampal CA1 region in senescence accelerated mouse
Jianzhong Li, Xinghua Hao, Huixian Cui, Zhibing Wu, Xuemin Liu, Ming Li, Haiping Wu
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Abstract
Objective
To explore the effects of enriched enviroment on neurons apoptosis in hippocampal CA1 region in senescence accelerated mouse.
Methods
Both twenty 5-month-old healthy male SAMP8 and twenty 5-month-old healthy male SAMR1 mice were randomly divided into enriched environment (EE) group and standard environment(SE) group, with 10 animals in each group. Mice in the 2 groups were subjected to the two different environments for 2 months. The number of neurons in hippocampal CA1 region was counted by Nissl staining. Apoptotic cells were detected by TUNEL staining. The deposition of β-amyloid(Aβ) in hippocampal CA1 was detected by immunohistochemical staining and image analysis system.
Results
For SAMP8 in EE group, the number of neurons was (96.656±2.444), the apoptotic index (AI) was (36.275%), the number of Aβ immune positive cell was (14.063±1.501) and the absorbance was (0.255±0.021). For SAMP8 in SE group, the number of neurons was (88.938±3.583), the AI was (51.961%), the number of Aβ immune positive cell was (17.844±1.648) and the absorbance was (0.291±0.032). For all indicators, there were significant difference between two groups (P 0.05).
Conclusion
Enriched environment can effectively reduce the deposition of Aβ, reduce the apoptosis of neurons, and prevent the damage and loss of neurons in hippocampal CA1 region in SAMP8 mice, which may be one of the mechanisms of enriched environment improving the learning and memory ability in mice.
Key words:
Enriched environment; Hippocampal CA1 region; Apoptosis; Alzheimer’s disease