Effects of captlpril on type I collagen in rat hepatic stellate cells

Objective To investigate the effects of captlpril on type Ⅰ collagen mRNA expression of culured hepatic stellate cells （ HSCs ） in vivo Methods HSC-T6 rat HSCs were chosen as the study model of the activated HSCs. Cultured HSCs were randomized into control group, angiotensin Ⅱ （ AngⅡ ） group （1 × 10^-5,1 ×10^-7mol/L） and Ang Ⅱ ＋ captlpril group （1 × 10^-5,1 ×10^-7mol/L）. HSCs were harvested to detect the collagen Ⅰ mRNA expression by reverse transcription-polymerase chain reaction （RT-PCR）. Results Type I collagen gene expression levels in different groups of AngⅡ （1 × 10^-5,1 ×10^-7mol/L） were 0. 850 ± 0. 107 and 0. 620 ±0. 103 respectively, and that in control group was 0. 438 ±0. 061. Type I collagen gene expression levels in different groups of Ang Ⅱ ＋ captlpril （ 1 × 10^-5,1 ×10^-7 mol/L） were 0.650 ±0.087 and 0.510 ±0.062. Conclusion Angll can increase the type I collage mRNA expression of HSCs, which was inhibited by captlpril. Key words: Hepatic stellate cells;  Angiotensin Ⅱ ;  Captlpril;  Type Ⅰ collagein