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Effects of extremely low frequency electromagnetic fields on the level of c-fos mRNA in brain and liver of mouse

刘赟, 喻云梅, 翁恩琪

Abstract

目的研究极低频电磁场暴露对小鼠脑和肝组织c-fos mRNA水平的影响.方法将小鼠暴露于50 Hz、0.2 mT及50 Hz、6.0 mT的电磁场中,持续2周或4周;采用竞争性RT-PCR方法检测小鼠脑和肝脏c-fos mRNA水平.结果50 Hz、0.2 mT及50 Hz、6.0 mT电磁场暴露2周后,小鼠脑组织c-fos mRNA的水平上升为(0.017 8±0.007 6)amol/120 ng cDNA和(0.009 2±0.004 2)amol/120 ng cDNA,与对照组[(0.001 2±0.000 5)amol/120 ng cDNA]比较,差异有显著性(P＜0.05);肝组织c-fos mRNA的水平上升为(0.0117±0.005 5)amol/120ng cDNA和(0.014 8±0.016 2)amol/120 ng cDNA,与对照组[(0.000 5±0.000 5)amol/120 ng cDNA]比较,差异有显著性(P＜0.05).0.2 mT和6.0 mT电磁场暴露4周后,小鼠脑组织c-fos mRNA的水平上升为(0.010 0±0.005 4)amol/120 ng cDNA和(0.019 8±0.007 9)amol/120 ng cDNA,与对照组[(0.001 5±0.000 8)amol/120ng cDNA]比较,差异有显著性(P＜0.05);肝组织c-fos mRNA的水平分别上升为(0.017 3±0.012 2)amol/120ng cDNA和(0.013 3±0.009 0)amol/120 ng cDNA,而对照组无表达.结论50 Hz电磁场暴露引起小鼠脑和肝脏c-fos基因转录水平明显上调.

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Key concepts: Complementary DNA, Molecular biology, Messenger RNA, Biology, Gene expression, Gene, Biochemistry