2001•中华放射医学与防护杂志Requires access
Studies on hematopoietic cell apoptosis and the relative gene expression in irradiated mouse bone marrow
Peng Yun, 王德文, 熊呈琦, 高亚兵, Yayao Hong, 崔玉芳, 汪宝珍
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Abstract
Objective To study apoptosis and expressions of bcl-2 and p53 in irradiated mouse bone marrow. Methods LACA mice were irradiated with 60Co γ-rays.By means of in situ terminal labeling,in situ hybridization and image analysis,the authors studied radiation-induced apoptosis of hematopoietic cells and the expressions of bcl-2 and p53. Results The characteristics of apoptosis appeared in hematopoietic cells at 6 hrs after irradiation.The expression of bcl-2 was obviously decreased when apoptosis of hematopoietic cells occurred,whereas it increased in the early recovery phase;p53 protein increased during both apoptosis of hematopoietic cells and the recovery phase,and mutant type p53 DNA was positive only in the recovery phase. Conclusion Radiation may induced apoptosis of hematopoietic cells in a dose-dependent manner;Both bcl-2 and p53 genes play an important role in apoptosis and recovery phase.