2009•Zhonghua jianyan yixue zazhiRequires access
Loop-mediated isothermal amplification and its application in virus detection
Xiaobo Bai, Hongqi Han, Fengwen Xu, Wentao Qiao
Open publisher page 0 citations
Abstract
Loop-mediated isothermal amplification ( LAMP ) , a novel isothermal nucleic acid amplification method, employs a DNA polymerase and four specially designed primers that recognize a total of six distinct sequences on the target DNA to amplify the target via stem-loop DNA structure. LAMP allows the synthesis of large amounts of DNA in a short period of time with high specificity under isothermal conditions. As the LAMP reaction progresses, the reaction by-product pyrophosphate ions bind to magnesium ions and form a white precipitate of magnesium pyrophosphate. Real-time turbidity measurements of the LAMP reaction make it to be a very useful molecular method for quantitutire analysis of nucleic acid.
Key words:
Nucleic acid amplification techniques; DNA viruses; RNA viruses