ScholarLens Back to search
2011•Journal of Pathogen BiologyRequires access

Use of loop-mediated isothermal amplification in parasitology research

Yang Yi-mei

Open publisher page 0 citations

Abstract

A novel method of nucleic acid amplification,termed loop-mediated isothermal amplification(LAMP),employs two pairs of specially designed primers and DNA polymerase with strand displacement activity.LAMP can efficiently amplify DNA with high specificity under isothermal conditions.The final products are stem-loop DNA with several inverted repeats of the target sequence and cauliflower-like structures with multiple loops.DNA synthesis is accompanied by a byproduct in the form of a white precipitate of magnesium pyrophosphate,providing a visible result.As a new technique for nucleic acid amplification,LAMP has been used in the study of parasitic diseases such as malaria and schistosomiasis.Research has shown that LAMP is sensitive,specific,and easy to perform.LAMP has a range of potential applications in parasitology research.

About this research paper

What this paper is about

A novel method of nucleic acid amplification,termed loop-mediated isothermal amplification(LAMP),employs two pairs of specially designed primers and DNA polymerase with strand displacement activity.LAMP can efficiently amplify DNA with high specificity under isothermal conditions.The final products are stem-loop DNA with several inverted repeats of the target sequence and cauliflower-like structures with multiple loops.DNA synthesis is accompanied by a byproduct in the form of a white precipitate of magnesium pyrophosphate,providing a visible result.As a new technique for nucleic acid amplification,LAMP has been used in the study of parasitic diseases such as malaria and schistosomiasis.Research has shown that LAMP is sensitive,specific,and easy to perform.LAMP has a range of potential applications in parasitology research.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A novel method of nucleic acid amplification,termed loop-mediated isothermal amplification(LAMP),employs two pairs of specially designed primers and DNA polymerase with strand displacement activity.LAMP can efficiently amplify DNA with high specificity under isothermal conditions.The final products are stem-loop DNA with several inverted repeats of the target sequence and cauliflower-like structures with multiple loops.DNA synthesis is accompanied by a byproduct in the form of a white precipitate of magnesium pyrophosphate,providing a visible result.As a new technique for nucleic acid amplification,LAMP has been used in the study of parasitic diseases such as malaria and schistosomiasis.Research has shown that LAMP is sensitive,specific,and easy to perform.LAMP has a range of potential applications in parasitology research.

Key concepts: Loop-mediated isothermal amplification, Nucleic acid, Biology, DNA, Applications of PCR, Ligase chain reaction, Recombinase Polymerase Amplification, Parasitology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Use of loop-mediated isothermal amplification in parasitology research — Research Paper | ScholarLens