HPLC analyses of polyphenolic compounds in oak (Querces frainetto) honey from Kırklareli region of Turkey

In this study, phenolic profile and total phenolic contents of oak honey, a kind of dark honeydew honey, were determined.The methanolic honey extract was enriched by liquid-liquid extraction with diethyl ether and ethyl acetate, and then was analyzed by RP-HPLC-UV with acetonitrile: water mobile phase.Nineteen phenolic standards were used to prepare calibration graphics.Seven phenolic acid (gallic acid, protocatequic acid, p-OH benzoic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid) and twelve flavonoids (catechin, epicatechin, rutin, myricetin, resveratrol, daidzein, luteolin, t-cinnamic acid, hesperetin, chrysin, pinocembrin, caffeic acid phenlyester (CAPE) were used.Total phenolic contents of the honey were measured by Folin Ciocalteau's assay.All of the polyphenols except epicatechin, rutin, luteolin, and hesperetin were detected in varying amounts.Protocatequic acid, ferulic acid, myricetin, and chrysin were the most abundant phenolic compounds.Total phenolic contents of the honey were from 54 to 88 mg GAE/100g.In summary, oak honey has high apitherapeutic value with rich polyphenol diversity.