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2020•Springer series in optical sciences/SPringer series in optical sciencesOpen access

Phase Microscopy to Enhance Contrast

Barry R. Masters

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Abstract

This chapter deals with imaging transparent objects (e.g., live unstained cells) in the light microscope. Most biological specimens are transparent (i.e., in the light microscope the image is of constant intensity across the field of view and there is no contrastContrast). Transparent specimens, such as thin layers of cells, are called phase objects.

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What this paper is about

This chapter deals with imaging transparent objects (e.g., live unstained cells) in the light microscope. Most biological specimens are transparent (i.e., in the light microscope the image is of constant intensity across the field of view and there is no contrastContrast). Transparent specimens, such as thin layers of cells, are called phase objects.

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OpenAlex reports 3 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

This chapter deals with imaging transparent objects (e.g., live unstained cells) in the light microscope. Most biological specimens are transparent (i.e., in the light microscope the image is of constant intensity across the field of view and there is no contrastContrast). Transparent specimens, such as thin layers of cells, are called phase objects.

Key concepts: Phase contrast microscopy, Microscope, Optical microscope, Microscopy, Optics, Bright-field microscopy, Materials science, Contrast (vision)

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