Effects of Different Hormone Concentrations on in Vitro Culture and Rooting of Fuchsia Hybrida
Yanrong Bai, Jiang Yalian, Jinying Wang
Abstract
Yanrong Bai, Jiang Yalian, Jinying Wang
Abstract
The effects of different plant growth regulators on the induction and differentiation of multiple shoot, multiplication of multiple shoot and rooting of multiple shoot in subculture were studied, adding different plant growth regulators 6-BA and NAA, different concentration and different proportion combination were studied. Results: the most suitable medium for induction was MS + 6-BA 0.1 mg / l + Sugar 30 G / L + Agar 6.2 g / L, the induction rate was 56.17% ; The most suitable medium for the multiplication of tufted buds of the plant was MS + 6-BA 1.0 mg / l + Naa 0.3 mg / l + Sugar 30 G / L + Agar 6.2 g / l, the Multiplication Coefficient was 4.07; The most suitable medium for rooting of tufted buds was 1 / 2MS + Naa 0.2 mg / l + sugar 30g / l + Agar 6.2 g / L, and the rooting rate was 100%.
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The effects of different plant growth regulators on the induction and differentiation of multiple shoot, multiplication of multiple shoot and rooting of multiple shoot in subculture were studied, adding different plant growth regulators 6-BA and NAA, different concentration and different proportion combination were studied. Results: the most suitable medium for induction was MS + 6-BA 0.1 mg / l + Sugar 30 G / L + Agar 6.2 g / L, the induction rate was 56.17% ; The most suitable medium for the multiplication of tufted buds of the plant was MS + 6-BA 1.0 mg / l + Naa 0.3 mg / l + Sugar 30 G / L + Agar 6.2 g / l, the Multiplication Coefficient was 4.07; The most suitable medium for rooting of tufted buds was 1 / 2MS + Naa 0.2 mg / l + sugar 30g / l + Agar 6.2 g / L, and the rooting rate was 100%.
Key concepts: Subculture (biology), Agar, Shoot, Sugar, Botany, Biology, Horticulture, Micropropagation