Optimization on the sulfated modification of polysaccharides from Coprinus comatus and evaluation of antioxidant activity

【Objective】The effects of Sulfated modification on Polysaccharides fromCoprinus comatus were studied to provide a basis for natural antioxidants'development.【Method】Polysaccharides fromC.comatus extracted by hot-water(WPC) was sulfated as the chlorosulfonic acid-Pyridine method.According to the degree of substitution,single factor experiment was operated to optimizing the sulfated modificiation.The effects of sulfated C.comatus polysaccharides(SWPC)on superoxide anion radicals and scavenging hydroxyl radicals were determined by pyrogallol autoxidation and phenanthroline-metal iron-H2O2 system respectively.【Result】The result showed that 1:3 volume ratio of chlorine sulfonic acid and pyridine,90℃,2hwere the optimum conditions.The effects on both scavenging hydroxyl radicals and superoxide anion radicals could be shown evidently.Compared with WPC,the effect of SWPC on scavenging hydroxyl radicals had been improved a lot,while diminished on scavenging superoxide anion radicals.【Conclusion】SWPC can scaveng both hydroxyl radicals and superoxide anion radicals,but the sensitive effects on them are quite different.