2014Indian Journal of Small Ruminants (The)Requires access

Seroprevalence of bluetongue virus antibodies in goats of Gujarat

A.G. Bhagat, B.S. Chandel, A.I. Dadawala, H.C. Chauhan, V.A. Pathan

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Abstract

In the present study, goat serum samples from different regions of Gujarat state were screened for the prevalence of BTV antibodies by using competitive enzyme linked immunosorbent assay (c-ELISA), indirect enzyme linked immunosorbent assay (i-ELISA) and agar gel immunodiffusion test (AGID). Out of the total 510 serum samples screened, 57.25%, 39.61% and 26.47% were found positive by cELISA, i-ELISA and AGID test, respectively. The results demonstrated a high prevalence of bluetongue virus antibodies in goats, providing serological evidence of widespread BTV infection in Gujarat state.

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What this paper is about

In the present study, goat serum samples from different regions of Gujarat state were screened for the prevalence of BTV antibodies by using competitive enzyme linked immunosorbent assay (c-ELISA), indirect enzyme linked immunosorbent assay (i-ELISA) and agar gel immunodiffusion test (AGID). Out of the total 510 serum samples screened, 57.25%, 39.61% and 26.47% were found positive by cELISA, i-ELISA and AGID test, respectively. The results demonstrated a high prevalence of bluetongue virus antibodies in goats, providing serological evidence of widespread BTV infection in Gujarat state.

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Available abstract

In the present study, goat serum samples from different regions of Gujarat state were screened for the prevalence of BTV antibodies by using competitive enzyme linked immunosorbent assay (c-ELISA), indirect enzyme linked immunosorbent assay (i-ELISA) and agar gel immunodiffusion test (AGID). Out of the total 510 serum samples screened, 57.25%, 39.61% and 26.47% were found positive by cELISA, i-ELISA and AGID test, respectively. The results demonstrated a high prevalence of bluetongue virus antibodies in goats, providing serological evidence of widespread BTV infection in Gujarat state.

Key concepts: Seroprevalence, Ouchterlony double immunodiffusion, Serology, Veterinary medicine, Antibody, Immunodiffusion, Virology, Virus

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