Hybrid identification of progenies of Zoysia crosses by SRAP marker.

Sequence-related Amplified Polymorphism(SRAP) is a new molecular marker technique developed on the basis of PCR.The authenticity of 37 progenies of 5 Zoysia grass cross-combinations were identified by SRAP marker to select out the true hybrids for further study.First,primers were selected to bands specific for the male parent but absent from the female,then 9 primers were selected and applied to identify the hybrids.The authenticity of the progenies was identified by whether or not they had the male parent specific bands.There were 32 progenies with these specific bands,which were therefore identified as true hybrids.The amplified bands of SRAP were clear and stable,and there was considerable variation in the hybrids,including some with new bands and some without the parent bands.The results suggest that SRAP markers could be used as effective molecular markers for the authenticity,identification and genetic analysis of the progenies of Zoysia grass