Authenticity Identification of Progenies from Interspecific Cross Red Globe (Vitis vinifera)×Shuangyou (Vitis amurensis) by SRAP Markers
Xiuwu Guo, Zhang Pengxiang, Guo YinShan, Zhendong Liu, Kun Li, Chengxiang Li
Abstract
Xiuwu Guo, Zhang Pengxiang, Guo YinShan, Zhendong Liu, Kun Li, Chengxiang Li
Abstract
Authenticity identification of interspecific cross progenies is necessary for the success of crossbreeding. The authenticity of 94 interspecific cross progenies from Red Globe×Shuangyou was identified by SRAP (Sequence—related Amplified Polymorphism) markers. Five paternal polymorphism primers were selected randomly between two parents by SRAP markers amplyfied and was applied to identify the authenticity of 94 progenies. The result showed that 87 progenies of 94 progenies were all identified to be true hybrids owing to their specific bands from male parent and morphological analysis of the field. Keywords Grape; SRAP marker; Authenticity identification
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Authenticity identification of interspecific cross progenies is necessary for the success of crossbreeding. The authenticity of 94 interspecific cross progenies from Red Globe×Shuangyou was identified by SRAP (Sequence—related Amplified Polymorphism) markers. Five paternal polymorphism primers were selected randomly between two parents by SRAP markers amplyfied and was applied to identify the authenticity of 94 progenies. The result showed that 87 progenies of 94 progenies were all identified to be true hybrids owing to their specific bands from male parent and morphological analysis of the field. Keywords Grape; SRAP marker; Authenticity identification
Key concepts: Biology, Interspecific competition, Vitis vinifera, Hybrid, Interspecific hybrids, Interspecific hybridization, Crossbreed, Identification (biology)