Extraction of total flavonoids from FRUCTUS LIGUSTRI LUCIDI.
Yan ZhenJun, Linghua Xu, Yaqing Xiong, Yang Yuqi, WU Xue-li, Wu XiJiao
Abstract
Yan ZhenJun, Linghua Xu, Yaqing Xiong, Yang Yuqi, WU Xue-li, Wu XiJiao
Abstract
[Objective] This study was to optimize the extraction process of total flavonoids from FRUCTUS LIGUSTRI LUCIDI using ethanol reflux method.[Method] On the basis of single factor test,orthogonal test L9(34) was designed to optimize the parameters related.[Result] The optimized extraction parameters were as follows:2.50 g dried powder of FRUCTUS LIGUSTRI LUCIDI as raw material,60% ethanol as extractant,solid-liquid ratio of 1:40,extraction temperature of 85 ℃,extraction duration of 2 h.Using these parameters the extraction yield of total flavonoids was as high as 6.215%.[Conclusion] Ethanol reflux method is stable and reliable in the extraction of total flavonoids from FRUCTUS LIGUSTRI LUCIDI.
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[Objective] This study was to optimize the extraction process of total flavonoids from FRUCTUS LIGUSTRI LUCIDI using ethanol reflux method.[Method] On the basis of single factor test,orthogonal test L9(34) was designed to optimize the parameters related.[Result] The optimized extraction parameters were as follows:2.50 g dried powder of FRUCTUS LIGUSTRI LUCIDI as raw material,60% ethanol as extractant,solid-liquid ratio of 1:40,extraction temperature of 85 ℃,extraction duration of 2 h.Using these parameters the extraction yield of total flavonoids was as high as 6.215%.[Conclusion] Ethanol reflux method is stable and reliable in the extraction of total flavonoids from FRUCTUS LIGUSTRI LUCIDI.
Key concepts: Extraction (chemistry), Chromatography, Chemistry, Yield (engineering), Ethanol, Raw material, Materials science, Biochemistry