Comparative Study of Anti-Bacterial Effect of Modified Chlorhexidine, Chlorhexidine and Sodium Hypochlorite on Enterococcus faecalis Bacteria in an Experimental Study

Sodium hypochlorite and chlorhexidine are materials that are usually used as root canal irrigants for disinfection of root canal and cleaning it. With adding surfactant sodium dodecyl sulfate modified chlorhexidine finds tissue solubility effect; thus in this study, we consider effect of ant property of this material on Enterococcus faecalis in experimental condition. Materials and methods: In this experimental study, pure colonies of Enterococcus faecalis were cultivated in BHI and then they were diluted. Next, CS 0.85% with turbidity rate of 0.5 MacFarlane was provided and then from 24 cell growth medium, 1 ml is added into each plate. 2 ml from each under tested solutions were mixed for 10 second by ultrasonic with suspension of bacteria and after certain times, then from each case, 1ml was taken and added into test tube in which fresh BHI was mixed with counteractive material of each irrigants. Finally, their turbidity rate was measured and then it was statistically analyzed. Results: There is no significant difference between chlorhexidine 1%, chlorhexidine 2%, chlorhexidine 2%, and modified chlorhexidine -0.2%. They all belong to one group. In addition, there is no significant difference between hip 5.5% and sodium hypochlorite 2.5%. However, there is significant difference. Conclusion: Results of the study indicated that using 2% SLS+ 0.2% CHXcan have similar effect as 1%-2% chlorhexidine and have 0.2% anti-bacterial effect.