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2019•International Journal of Current Microbiology and Applied SciencesOpen access

Molecular Detection of Peste des-petits ruminant virus (PPRV) by RT-PCR

Pragati Bhoyar, P.A. Tembhurne, Ruchi Bhate, Prashant Tarale, Anuradha Nemade, Anand Kadam, Jivan Kesharkar, VC Ingle

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Abstract

Peste des petits ruminants is one of the important transboundary, highly contagious and infectious viral disease of small ruminants especially sheep and goats caused by Small Ruminant Morbillivirus alias Peste- des- petits ruminants virus. In the current study, PPRV was detected in clinical samples of suspected goats as well as post vaccinated samples in nasal swabs and PBMC by conventional RT-PCR. Out of the 23 nasal swabs (12 infected and 11 vaccinated) 11 infected and one vaccinated samples was found positive. Out of 16 (5 infected and 11 vaccinated), all five infected and one vaccinated PBMC samples were found positive. Thus, the present study delineated the presence of PPRV in clinical samples as well as viaremic stages of PPRV. Further sequencing and characterization will warrant lineage of PPRV circulating in Nagpur district of Maharashtra.

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Peste des petits ruminants is one of the important transboundary, highly contagious and infectious viral disease of small ruminants especially sheep and goats caused by Small Ruminant Morbillivirus alias Peste- des- petits ruminants virus. In the current study, PPRV was detected in clinical samples of suspected goats as well as post vaccinated samples in nasal swabs and PBMC by conventional RT-PCR. Out of the 23 nasal swabs (12 infected and 11 vaccinated) 11 infected and one vaccinated samples was found positive. Out of 16 (5 infected and 11 vaccinated), all five infected and one vaccinated PBMC samples were found positive. Thus, the present study delineated the presence of PPRV in clinical samples as well as viaremic stages of PPRV. Further sequencing and characterization will warrant lineage of PPRV circulating in Nagpur district of Maharashtra.

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Available abstract

Peste des petits ruminants is one of the important transboundary, highly contagious and infectious viral disease of small ruminants especially sheep and goats caused by Small Ruminant Morbillivirus alias Peste- des- petits ruminants virus. In the current study, PPRV was detected in clinical samples of suspected goats as well as post vaccinated samples in nasal swabs and PBMC by conventional RT-PCR. Out of the 23 nasal swabs (12 infected and 11 vaccinated) 11 infected and one vaccinated samples was found positive. Out of 16 (5 infected and 11 vaccinated), all five infected and one vaccinated PBMC samples were found positive. Thus, the present study delineated the presence of PPRV in clinical samples as well as viaremic stages of PPRV. Further sequencing and characterization will warrant lineage of PPRV circulating in Nagpur district of Maharashtra.

Key concepts: Peste-des-petits-ruminants virus, Peste-des-Petits-Ruminants, Morbillivirus, Virology, Biology, Ruminant, Virus, Viral disease

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