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2019•PubMedRequires access

[Preliminary study on the effect of lipopolysaccharide challenge on the genes expression of inflammatory factors in A549 cells].

Tengsong Zhang, Qiao Sun, Jie Di, Keke Wang, Yan Qu

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Abstract

OBJECTIVE: To explore the effects of lipopolysaccharide (LPS) on the expression of inflammatory genes in A549 cells line under different concentrations and different action time, this study laid the foundation for further establishment of acute respiratory distress syndrome (ARDS) cell model in the optimal concentration-time way. METHODS: method. RESULTS: ): 5.57±0.25 vs. 1.00±0.00, all P < 0.01], there were no significant changes in the expression levels of inflammatory genes in A549 cells line of other concentration groups. CONCLUSIONS: The LPS concentration of 10 mg/L and the action time of 1 hour are the most suitable concentration-time conditions for establishing ARDS cell models of A549 cells line.

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What this paper is about

OBJECTIVE: To explore the effects of lipopolysaccharide (LPS) on the expression of inflammatory genes in A549 cells line under different concentrations and different action time, this study laid the foundation for further establishment of acute respiratory distress syndrome (ARDS) cell model in the optimal concentration-time way. METHODS: method. RESULTS: ): 5.57±0.25 vs. 1.00±0.00, all P < 0.01], there were no significant changes in the expression levels of inflammatory genes in A549 cells line of other concentration groups. CONCLUSIONS: The LPS concentration of 10 mg/L and the action time of 1 hour are the most suitable concentration-time conditions for establishing ARDS cell models of A549 cells line.

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Available abstract

OBJECTIVE: To explore the effects of lipopolysaccharide (LPS) on the expression of inflammatory genes in A549 cells line under different concentrations and different action time, this study laid the foundation for further establishment of acute respiratory distress syndrome (ARDS) cell model in the optimal concentration-time way. METHODS: method. RESULTS: ): 5.57±0.25 vs. 1.00±0.00, all P < 0.01], there were no significant changes in the expression levels of inflammatory genes in A549 cells line of other concentration groups. CONCLUSIONS: The LPS concentration of 10 mg/L and the action time of 1 hour are the most suitable concentration-time conditions for establishing ARDS cell models of A549 cells line.

Key concepts: Medicine, Lipopolysaccharide, A549 cell, Gene expression, Molecular biology, Tumor necrosis factor alpha, Cell culture, Andrology

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[Preliminary study on the effect of lipopolysaccharide challenge on the genes expression of inflammatory factors in A549 cells]. — Research Paper | ScholarLens