[Effects of hnRNP B, siRNA on the expression of p53 in human lung adenocarcinoma cell line A549].

OBJECTIVE: To explore the effects of hnRNP B1 on the expression of p53 in human lung adenocarcinoma cell line A549. METHODS: Two lines of A549 cells transfected by hnRNP B1 specific siRNA (recombinant plasmid A and D), which had a high transfection efficiency, were established as experimental groups. One line of A549 cells and one line of A549 cells transfected by pure plasmid served as controls. All of the cells were collected after treated with 10 micromol/L cisplatin for 24 h. The expressions of p53 mRNA and protein were detected by real-time RT-PCR and Western blot analysis. The expression of Phospho-P53 protein was detected by western blot analysis. RESULTS: No significant differences in the expression of p53 mRNA and protein among the groups were found. The expression ratio of Phospho-P53 protein in the cells transfected by hnRNP B1 siRNA were 0.87 +/- 0.06 and 0.75 +/- 0.06, which were significantly greater than those in the A549 cell (0.21 +/- 0. 04) and in the A549 cells transfected by pure plasmid (0.30 +/- 0.08) (P < 0.01). CONCLUSION: hnRNP B, specific siRNA can up regulate the activity of p53 in A549 cells.