Cross adaptive response of human blood lymphocytes in vitro, bone marrow cells and germ cells in mice in vivo induced by low doses of X-rays and low concentrations of MMC, H_(2)O_(2) and CP

Interaction of low doses of X-rays (two doses of 10 mGy and 50 mGy, a dose rate of 50 mGy)min) and low concentrations of MMC, H_2O_2 and CP on chromosome aberrations of human blood lymphocytes in vitro, bone marrow cells and germ cells in mice in vivo was studied in order to elucidate cross adaptive response between radiation and chemical agents. Results showed that cross adaptive response could be induced of (1) chromosome aberrations of bone marrow cells when MMC with different concentration (0.5, 2.5 and 5.0 mg/kg) were i.p. injected into mice 3 h after irradiation to 10 mGy X-rays. (2) chromosome aberrations of germ cells in mice by radiation and MMC, H_2O_2 when different concentrations of MMC, H_2O_2 and CP were i.p. injected into mice 3 h after irradiation to 50 mGy X-rays. However, it is not the case when CP applied, there is the synergetic effect between radiation and CP. (3) chromosome aberrations of human blood lymphocytes by 1.5 Gy X-irradiation 6 h after MMC (final concentration is 35 mg/mL) applied. (4) Anti-X rays damage by 1.5 Gy X-irradiation 24 h after low concentrations of MMC and H_2O_2, while not by CP. It provided the same conclusion as (2). The results suggested that the cross adaptive response of X-rays and MMC was induced not only in bone marrow cells in mice, but also in human blood lymphocytes in vitro. Besides, the response of interaction could be induced in germ cells in mice in vivo between low doses of X-rays and low concentrations of MMC and H_2O_2. But it is not the case for low concentrations of CP, the synergetic effect was produced between low concentration of CP and low dose of X-rays.