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Adaptive Response of Murine Thymocytes Induced by Low Dose γ-rays before MMC Exposure

Meiyi Xu

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Abstract

Objective:To observe the mechanism against MMC damage induced by low dose γ-rays on thymocytes in mice.Methods: The cycle progression and DNA contents of thymocytes were detected with flow cytometry and 3H-TdR 6 h after low dose radiation in male Kunming mice.Results: Compared with the simple use of MMC, The rates of spontaneous proliferation of thymocytes and S phase cell percentage significantly increased at the dose of 50 and 100 mGy combined with MMC(P0.01, P0.05).The percentage of G0/G1 phase cells was significantly decreased in irradiated and MMC group compared with that in simple MMC group.The percentage of G2/M phase cells was significantly reduced at dose of 100 mGy on thymocytes in irradiated and MMC group compared with that in simple MMC group.Conclusions: Low dose γ-rays effectively stimulates the synthesis of DNA and accelerates cell cycle progression on thymocytes in mice.

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Objective:To observe the mechanism against MMC damage induced by low dose γ-rays on thymocytes in mice.Methods: The cycle progression and DNA contents of thymocytes were detected with flow cytometry and 3H-TdR 6 h after low dose radiation in male Kunming mice.Results: Compared with the simple use of MMC, The rates of spontaneous proliferation of thymocytes and S phase cell percentage significantly increased at the dose of 50 and 100 mGy combined with MMC(P0.01, P0.05).The percentage of G0/G1 phase cells was significantly decreased in irradiated and MMC group compared with that in simple MMC group.The percentage of G2/M phase cells was significantly reduced at dose of 100 mGy on thymocytes in irradiated and MMC group compared with that in simple MMC group.Conclusions: Low dose γ-rays effectively stimulates the synthesis of DNA and accelerates cell cycle progression on thymocytes in mice.

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Available abstract

Objective:To observe the mechanism against MMC damage induced by low dose γ-rays on thymocytes in mice.Methods: The cycle progression and DNA contents of thymocytes were detected with flow cytometry and 3H-TdR 6 h after low dose radiation in male Kunming mice.Results: Compared with the simple use of MMC, The rates of spontaneous proliferation of thymocytes and S phase cell percentage significantly increased at the dose of 50 and 100 mGy combined with MMC(P0.01, P0.05).The percentage of G0/G1 phase cells was significantly decreased in irradiated and MMC group compared with that in simple MMC group.The percentage of G2/M phase cells was significantly reduced at dose of 100 mGy on thymocytes in irradiated and MMC group compared with that in simple MMC group.Conclusions: Low dose γ-rays effectively stimulates the synthesis of DNA and accelerates cell cycle progression on thymocytes in mice.

Key concepts: Flow cytometry, Cell cycle, Irradiation, DNA synthesis, DNA damage, Cell cycle progression, Andrology, Thymocyte

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