ScholarLens Back to search
2019•IOP Conference Series Materials Science and EngineeringOpen access

Determination of Total Flavonoids in Watermelon by Spectrophotometry

Yin Wei, Xiaotong Wu, Jinling Yang, Kai Zhang

Open full text 4 citations

Abstract

Objective to explore a method for the determination of total flavonoids in watermelon by spectrophotometry. Method: 100g watermelon juice was taken, centrifuged (3500r/min 20min) to obtain sample solution for use, and rutin was used as standard to measure the absorbance of watermelon sample solution at 360nm. Results: The regression equation is y=0.0227x+0.0766. The correlation coefficient is R2=0.9919, and the sample concentration is in the range of 30ug/mL-60ug/mL. The measured absorbance value has a good correlation with the concentration, stability and weight. The present is higher. Conclusion: Spectrophotometry is a widely used method, which has the characteristics of simple and rapid operation, and the required reagents are relatively simple and easy to obtain. Therefore, spectrophotometry can be one of the methods for determining the total flavonoid content in watermelon.

Open-access reader

About this research paper

What this paper is about

Objective to explore a method for the determination of total flavonoids in watermelon by spectrophotometry. Method: 100g watermelon juice was taken, centrifuged (3500r/min 20min) to obtain sample solution for use, and rutin was used as standard to measure the absorbance of watermelon sample solution at 360nm. Results: The regression equation is y=0.0227x+0.0766. The correlation coefficient is R2=0.9919, and the sample concentration is in the range of 30ug/mL-60ug/mL. The measured absorbance value has a good correlation with the concentration, stability and weight. The present is higher. Conclusion: Spectrophotometry is a widely used method, which has the characteristics of simple and rapid operation, and the required reagents are relatively simple and easy to obtain. Therefore, spectrophotometry can be one of the methods for determining the total flavonoid content in watermelon.

Why it matters

OpenAlex reports 4 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective to explore a method for the determination of total flavonoids in watermelon by spectrophotometry. Method: 100g watermelon juice was taken, centrifuged (3500r/min 20min) to obtain sample solution for use, and rutin was used as standard to measure the absorbance of watermelon sample solution at 360nm. Results: The regression equation is y=0.0227x+0.0766. The correlation coefficient is R2=0.9919, and the sample concentration is in the range of 30ug/mL-60ug/mL. The measured absorbance value has a good correlation with the concentration, stability and weight. The present is higher. Conclusion: Spectrophotometry is a widely used method, which has the characteristics of simple and rapid operation, and the required reagents are relatively simple and easy to obtain. Therefore, spectrophotometry can be one of the methods for determining the total flavonoid content in watermelon.

Key concepts: Absorbance, Rutin, Spectrophotometry, Chemistry, Chromatography, Correlation coefficient, Reagent, Analytical Chemistry (journal)

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Total Flavonoids in Watermelon by Spectrophotometry — Research Paper | ScholarLens