Construction and Expression of Eukaryotic Recombinant Plasmid Including CAV VP3 Gene and Human IL-18 Gene and Influence on Colon Cancer Cell

IRES promotor and neomycin gene from pIRES1 vector were inserted into multiple clone site of pVAX1 vector, then a recombinant plasmid pWP3IL-18 was constructed by inserting CAV VP3 gene and human IL-18 gene into multiple clone site and neomycin site , respectively. The recombinant plasmid pVVP3IL-18 was transfected to Hela cells and the results of indirect immunofluorescence showed that specific bright yellow green flurescene was observed around the membrane and across the plasma of the transfected Hela cells when tested with anti-mouse Ig G, FITC conjugate. The results of AO/EB staining and electron microscope showed apoptosis was induced greatly after pVVP3IL-18 was transfected to HCT-116 cells. The above results showed that the recombinant plasmid pVVP3IL-18 can express in Hela cells and can induce apoptosis in HCT-116 cells.