The Role of Annexin A1 in the Carcinogensis of Breast Cancer

Objective To detect the expression of Annexin A1 in normal breast tissues,ductal hyperplasia,non-invasive carcinoma and invasive carcinoma.Methods The immunohistochemistry SABC method and Western blot were used to determine the expression of Annexin A1 in 120 cases of normal breast tissues,ductal hyperplasia,non-invasive carcinoma and invasive carcinoma.(Results) The average score of Annexin A1 staining was 1.5328±0.45603 in normal breast tissue,1.3250±0.56928 in hyperplasia,0.9125±0.40324 in precancerous lesions,and only 0.1550±0.20125 and 0.1038±0.12004 in non-invasive and invasive carcinomas,respectively(Table1).There was no significant difference for annexin A1 staining between normal and ductal hyperplasia(P=(0.907)) and between non-invasive and invasive carcinoma(P=0.973).A significant loss of annexin A1 staining was observed both in breast cancer versus normal breast epithelium(P0.0001) and ductal hyperplasia(P0.0001),as well as precancerous lesions versus normal breast epithelium(P0.0001),ductal hyperplasia(P0.0001),and breast cancer(P0.0001).Moreover,the decrease espression of annexin A1 was correlated with clinical stage and tumor size(P0.05) and not with the age and lymph node metastasis(P0.05).The results by Western blot was the same as above.Conclusion Annexin A1 may play an important role in early diagnosis of breast cancer and in predicting the risk of carcinogenesis from precancerous lesions.