Effect and mechanism of action of aspirin induced apoptosis of human cervical cancer cell lines Hela

OBJECTIVE To study the regulatory effect of aspirin on the cell apoptosis of human cervical cancer cell lines Hela and its molecular mechanism of action.METHODS Hela cells were cultured in vitro and treated with aspirin at the concentration of 0.5,1.0,5.0 mmol·L-1respectively.The rate of apoptosis and cell cycle were analyzed by flow cytometry analysis(FACS).Western-blot was performed to examine the expression of NF-κB p65 protein.RESULTS 0.5,1.0,5.0 mmol·L-1 aspirin significantly increased the rate of apoptosis and the proportion of cells in G0/G1 phase and decreased the proportion of cells in S and G2/M phases in a dose-depending manner.With the increasing of aspirin concentration,the expression of NF-κB p65 was gradually downregulated.CONCLUSION Aspirin can regulate cell cycle distribution and accelerate cell apoptosis through inhibiting the expression of NF-κB p65 protein in cell lines Hela.